The UK Health Security Agency (UKHSA) confirmed 43 of the new patients were in England, while one case was spotted in each Scotland and Wales.
No further details were given but 'most cases' continue to be among gay and bisexual men, officials said.
Since the first case was detected on May 6, 348 people have been diagnosed with the tropical virus in England, 12 in Scotland, four in Wales and two in Northern Ireland.
The UK has recorded the most cases in the worldwide cluster, followed by Spain (259), Portugal (191) and Germany (150).
More than 1,300 infections have been confirmed worldwide.
